Micellar electrokinetic capillary chromatography of psoralen and isopsoralen

被引:2
|
作者
Wang, DX
Yang, GL [1 ]
Engelhardt, H
Zhang, HY
机构
[1] Hebei Univ, Dept Chem, Baoding 071002, Peoples R China
[2] Univ Saarlandes, Inst Analyt Umweltanalyt, D-6600 Saarbrucken, Germany
关键词
micellar electrokinetic chromatography; psoralen; isopsoralen;
D O I
10.1002/(SICI)1522-2683(19990701)20:9<1895::AID-ELPS1895>3.3.CO;2-C
中图分类号
Q5 [生物化学];
学科分类号
071010 ; 081704 ;
摘要
In this paper, a micellar electrokinetic capillary chromatography (MEKC) method to analyze the active components of psoralen and isopsoralen in the Chinese herb Psoralea corylifolia L. was developed. It was found that the optimum separation condition for the analysis of these compounds was a buffer solution which contained phosphate, berate and sodium dodecyl sulfate (pH 9.2). Through optimization of the experimental condition, the two isomers could be baseline-separated and their contents in the methanol-extracted sample were quantitatively analyzed.
引用
收藏
页码:1895 / 1899
页数:5
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