Molecular analysis of endochitinase gene cloned from Trichoderma spp.

被引：0
|
作者：
Bhat, Ganapati [1 ]
Bhat, Sumangala [1 ]
Kuruvinashetti, M. S. [1 ]
机构：
[1] Univ Agr Sci, Dept Biotechnol, Dharwad 580005, Karnataka, India
来源：
INDIAN JOURNAL OF GENETICS AND PLANT BREEDING | 2008年 / 68卷 / 01期
关键词：
Trichoderma; chitinase; cloning; phylogenetic analysis;
D O I：
暂无
中图分类号：
Q94 [植物学];
学科分类号：
071001 ;
摘要：
In this study, genes encoding endochitinase (chit46) were cloned from two native species of Trichoderma viz., Trichoderma viride IABT1012 and Trichoderma harzianum IABT1015 through PCR amplification using specific primers. The nucleotide sequence of cloned gene indicated the presence of reading frame of 1290 nucleotides. Similar to other chitinases these chitinase genes have chitinase active site (FDGIDVDWE), putative chitin binding site (XXXSXGG), 21 amino acid signal peptide and secondary processing site at 32-33(rd) amino acid position. Phylogenetic analysis indicated that these two genes fall in a same cluster and more related to reported T. harzianum endochitinase.
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页码：68 / 72
页数：5
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