Simultaneous Determination of 33 Medicine Residues in Aquatic Products by Rapid Resolution Liquid Chromatography-Tandem Mass Spectrometry

A new approach for the simultaneous determination of five classes residues, tetracyclines, quinolones, sulfoamides, tripmethoprim and triphenylmethane, in aquatic products by rapid resolution liquid chromatography-tandem mass spectrometry (RRLC-MS/MS) was established. Analytes were extracted by Na(2)EDTA-Mcllvaine buffer and acetonitrile, then defatted using hexane. Electrospray ionization mass spectrometry was operated in the positive mode using dynamic multiple reaction monitoring (DMRM) for the qualitative and quantitative analysis of 33 analytes after the separation on RRLC. The correlation coefficients of linear calibration curves were over 0.99 in the corresponding concentration range. The average recoveries of the 33 analytes ranged from 63.6% to 115.2%,and the relative standard deviation (RSD) in three different concentrations was 4.6%-14.6%. The limit of detection (LOD, S/N >= 3) and quantification (LOQ, S/N >= 10) were 0.1-2.0 mu g/kg and 0.5-5.0 mu g/kg, respectively. The method is simple, fast, sensitive and reliable, and is suitable for the determination of residues in aquatic products.