Dyella solisilvae sp. nov., isolated from mixed pine and broad-leaved forest soil

A Gram-stain-negative, aerobic, motile, yellow-pigmented, rod-shaped with a single polar flagellum bacterial strain, designated strain DHG54(T), was isolated from a forest soil sample of Dinghushan Biosphere Reserve, Guangdong Province, China. Strain DHG54(T) grew at 12-37 degrees C (optimum, 28 degrees C), pH 4.5-8.0 (optimum, pH 6.0-7.0) and in the presence of 0-3.0 % (w/v) NaCl (optimum, 0-1.5 %, w/v). Based on 16S rRNA gene sequence analysis, strain DHG54(T) formed a Glade with the members of the genus Dyella and showed highest sequence similarities of 98.2 % to Dyella japonica DSM 16301(T) and Dyella terrae KACC 12748(T). This was also supported by phylogenetic analysis based on the concatenated partial gyrB, lepA and recA housekeeping gene sequences. DNA-DNA hybridization results between strain DHG54(T) and closely related Dyella species were all lower than 70 %. Ubiquinone-8 was the only respiratory quinone, and iso-C-15:0 , iso-C-17:0 and iso-C-17(:)1 omega 9c were major fatty acids. The DNA G+C content of strain DHG54(T) was 65.4 mol%. The major polar lipids were phosphatidylethanolamine, phosphatidylglycerol and diphosphatidylglycerol. On the basis of the polyphasic characterization results presented here, strain DHG54(T) represents a novel species of the genus Dyella, for which the name Dyella solisilvae sp. nov. (type strain DHG54(T)=GDMCC 1.1187(T) = LMG 30091(T) ) is proposed.