Identification, Classification and Characterization of LBD Transcription Factor Family Genes in Pinus massoniana

被引:5
|
作者
Zhang, Chi [1 ]
Zhu, Peihuang [1 ]
Zhang, Mengyang [1 ]
Huang, Zichen [1 ]
Hippolyte, Agassin Romaric [1 ]
Hou, Yangqing [1 ]
Lou, Xuan [1 ]
Ji, Kongshu [1 ]
机构
[1] Nanjing Forestry Univ, Coinnovat Ctr Sustainable Forestry Southern China, Key Lab Forestry Genet & Biotechnol, Minist Educ, Nanjing 210037, Peoples R China
基金
国家重点研发计划;
关键词
lateral organ boundaries domain; Pinus massoniana; gene expression; pine wood nematode; GENOME-WIDE ANALYSIS; LATERAL ROOT; ARABIDOPSIS; SUSCEPTIBILITY; PROTEINS; MEMBERS; GROWTH;
D O I
10.3390/ijms232113215
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
Transcription factors (TFs) are a class of proteins that play an important regulatory role in controlling the expression of plant target genes by interacting with downstream regulatory genes. The lateral organ boundary (LOB) structural domain (LBD) genes are a family of genes encoding plant-specific transcription factors that play important roles in regulating plant growth and development, nutrient metabolism, and environmental stresses. However, the LBD gene family has not been systematically identified in Pinus massoniana, one of the most important conifers in southern China. Therefore, in this study, we combined cell biology and bioinformatics approaches to identify the LBD gene family of P. massoniana by systematic gene structure and functional evolutionary analysis. We obtained 47 LBD gene family members, and all PmLBD members can be divided into two subfamilies, (Class I and Class II). By treating the plants with abiotic stress and growth hormone, etc., under qPCR-based analysis, we found that the expression of PmLBD genes was regulated by growth hormone and abiotic stress treatments, and thus this gene family in growth and development may be actively involved in plant growth and development and responses to adversity stress, etc. By subcellular localization analysis, PmLBD is a nuclear protein, and two of the genes, PmLBD44 and PmLBD45, were selected for functional characterization; secondly, yeast self-activation analysis showed that PmLBD44, PmLBD45, PmLBD46 and PmLBD47 had no self-activating activity. This study lays the foundation for an in-depth study of the role of the LBD gene family in other physiological activities of P. massoniana.
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页数:18
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