New Alternative of Enhancing Hospital Hygiene Facing Pseudomonas aeruginosa Drug Resistance Impact of Hypertonic Saline Solutions on the Behavior of P. aeruginosa

The objective of this study was to elucidate the antibacterial effect of hypertonic saline of KH2PO4 and NaCl solutions for enhancing hospital hygiene facing drug resistance of the biofilm of Pseudomonas aeruginosa isolated from a hospital environment on an agar cetrimide medium. The variations of P. aeruginosa biofilm density were measured following optical surface density by a correlated imaging method. Inhibition tests of 90% of the biofilm in a selective liquid medium monitored by spectrophotometry showed inhibition rates of 91.2 and 91.1% for minimal biofilm inhibition concentrations of 5.4 and 3.6% for NaCl and KH2PO4, respectively. The results of the eradication tests of 50% of the biofilm showed minimal biofilm eradication concentrations of 6.3 and 3.6% and eradication ratios of 50.6 and 50.9% for NaCl and KH2PO4, respectively. In the case of a selective solid medium, the results exhibited a minimal biofilm inhibition concentration of 4.2 and 3.9%, a biofilm inhibition rate of 90.8 and 90.5%, and a minimal biofilm eradication concentration of 2.7 and 3.3% for a biofilm killing quotient of 55.0 and 61.2% in NaCl and KH2PO4 cases, respectively. Accordingly, the results obtained demonstrated the potential of the hypertonic saline solutions and suggested that the employment of these solutions in hospital hygiene can be an alternative of prevention of nosocomial infections by P. aeruginosa.