Anti-proliferative action of chrysin in colon cancer cells and its effects on signaling pathways

The goal of the current study is to elucidate the anticancer potential of chrysin against a panel of colon HT-55, HCA-7 and LoVo cancer cell lines. The anti-proliferation effects were checked out using MTT and BrDu incorporation assays against all the three cell lines. The inhibition in the case of HT-55, HCA-7 and LoVo cell lines with IC50 values of 0.4 mM, 0.4 mM and 0.8 mM were obtained. In addition DNA synthesis were also observed with IC50 values of 2.5, 4.2 and 1.8 mM for HT-55, HCA-7 and LoVo cell lines, respectively. Based on the efficient activity in anti-proliferation methods, HT-55 cells were chosen to carry out further experimental studies. Various changes in signaling pathways of HT-55 cells were observed upon chrysin treatment. Changes in ERK1/2, p38, CREB and AKT were observed while testing HT-55 cells with chrysin. In vitro studies of chrysin in colon cancer cells has also been elucidated.