Comparison of vitrification and conventional freezing for cryopreservation of caprine embryos

被引：8
|
作者：
Araujo-Lemos, Paula F. B.
Freitas Neto, Leopoldo M.
Moura, Marcelo T.
Melo, Janana V.
Lima, Paulo F.
Oliveira, Marcos A. L. [1 ]
机构：
[1] Univ Fed Rural Pernambuco UFRPE, Dept Vet Med, Lab Biotecn Aplicadas Reprod Anim, BR-52171900 Recife, PE, Brazil
来源：
ZYGOTE | 2015年 / 23卷 / 04期
关键词：
Blastocyst; Cryobiology; Cryoprotectant; Goat; Slow freezing; PULLED-STRAW METHOD; GOAT EMBRYOS; MOUSE EMBRYOS; SURVIVAL; SEMEN; DIMETHYLFORMAMIDE; FROZEN; SPERM; BLASTOCYSTS; GLYCEROL;
D O I：
10.1017/S0967199414000215
中图分类号：
Q2 [细胞生物学];
学科分类号：
071009 ; 090102 ;
摘要：
The experiment aimed to compare conventional freezing and different vitrification protocols for cryopreservation of caprine embryos at morphological, ultrastructural, and functional levels. Caprine embryos produced in vivo were allocated randomly to three groups: (1) conventional freezing with ethylene glycol (EG); (2) dimethyl sulfoxide + EG (DMSO/EG) vitrification; and (3) dimethylformamide + EG (DMF/EG) vitrification. All groups were scored for cell viability (propidium iodide staining and ultrastructural levels) and re-expansion rate after thawing or warming. Embryos subjected to DMSO/EG vitrification showed higher cell viability (73.33%), compared with DMF/EG vitrification and conventional freezing group embryos (40.00 and 66.66%, respectively). The ultrastructural study revealed that vitrified embryos had greater preservation of cellular structure than embryos from conventional freezing with EG. DMSO/EG vitrification resulted in higher rates of re-expansion in vitro (47.36%) than DMF/EG vitrification (31.58%), and conventional freezing (25.00%). In conclusion, caprine embryos produced in vivo are better cryopreserved after vitrification than conventional freezing, therefore we conclude that DMSO/EG vitrification is the most effective protocol for cryopreservation.
引用
收藏
页码：594 / 602
页数：9
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