First detection of SARS-CoV-2 RNA in wastewater in North America: A study in Louisiana, USA

被引：372
|
作者：
Sherchan, Samendra P. [1 ]
Shahin, Shalina [1 ]
Ward, Lauren M. [1 ]
Tandukar, Sarmila [2 ]
Aw, Tiong G. [1 ]
Schmitz, Bradley [3 ]
Ahmed, Warish [4 ]
Kitajima, Masaaki [5 ]
机构：
[1] Tulane Univ, Dept Environm Hlth Sci, 1440 Canal St,Suite 2100, New Orleans, LA 70112 USA
[2] Univ Yamanashi, Interdisciplinary Ctr River Basin Environm, 4-3-11 Takeda, Kofu, Yamanashi 4008511, Japan
[3] Loudoun Water, 44865 Loudoun Water Way, Ashburn, VA 20147 USA
[4] CSIRO Land & Water, Ecosci Precinct, 41 Boggo Rd, Dutton Pk, Qld 4102, Australia
[5] Hokkaido Univ, Fac Engn, Div Environm Engn, Kita Ku, North 13 West 8, Sapporo, Hokkaido 0608628, Japan
来源：
SCIENCE OF THE TOTAL ENVIRONMENT | 2020年 / 743卷
基金：
日本学术振兴会;
关键词：
Wastewater-based epidemiology; SARS-CoV-2; Surveillance; COVTD-19; RT-qPCR; Wastewater; REAL-TIME PCR; CORONAVIRUS;
D O I：
10.1016/j.scitotenv.2020.140621
中图分类号：
X [环境科学、安全科学];
学科分类号：
08 ; 0830 ;
摘要：
We investigated the presence of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) RNA in wastewater samples in southern Louisiana, USA. Untreated and treated wastewater samples were collected on five occasions over a four-month period from January to April 2020. The wastewater samples were concentrated via ultrafiltration (Method A). and an adsorption-elution method using electronegative membranes (Method B). SARS-CoV-2 RNA was detected in 2 out of 15 wastewater samples using two reverse transcription-quantitative polymerase chain reaction (RT-qPCR) assays (CDC N1 and N2). None of the secondary treated and final effluent samples tested positive for SARS-CoV-2 RNA. To our knowledge, this is the first study reporting the detection of SAR.S-CoV-2 RNA in wastewater in North America, including the USA. However, concentration methods and RTqPCR assays need to be refined and validated to increase the sensitivity of SARS-CoV-2 RNA detection in wastewater. (C) 2020 Elsevier B.V. All rights reserved.
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页数：6
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