Activity of Pichia pastoris alternative cis-prenyltransferase is correlated with proliferation of peroxisomes

被引：3
|
作者：
Skoneczny, M [1 ]
Kludkiewicz, B [1 ]
Swiezewska, E [1 ]
Szkopinska, A [1 ]
机构：
[1] PAS, Inst Biochem & Biophys, PL-02106 Warsaw, Poland
来源：
CELL BIOLOGY INTERNATIONAL | 2006年 / 30卷 / 02期
关键词：
cis-prenyltransferases; peroxisomes; dolichols; methylotrophic yeast;
D O I：
10.1016/j.cellbi.2005.09.006
中图分类号：
Q2 [细胞生物学];
学科分类号：
071009 ; 090102 ;
摘要：
We have investigated dolichol synthesis in yeast Pichia pastoris. Growth of these cells on methanol causes peroxisome proliferation and induction of peroxisomal enzymes. Twenty-four hours methanol treatment was sufficient for the appearance of longer-chain dolichols. Less specific oleic acid induction needed 48 h for the synthesis of longer dolichol family with typical one still present. Cells cultured in non-inducing conditions for 48 h did not reveal the presence of additional dolichol family. Peroxisomes purified from oleic acid treated cells synthesize in vitro polyprenols longer by two isoprene residues than those synthesized by microsomal fraction from glucose culture. These observations lead us to suggest that chain length of dolichols synthesized in yeast cell may depend on the carbon and energy source supply which mobilizes metabolic pathways localized to different cellular compartments. (c) 2005 International Federation for Cell Biology. Published by Elsevier Ltd. All rights reserved.
引用
收藏
页码：122 / 126
页数：5
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  • [1] Activity of Pichia pastoris alternative cisprenyltransferase is correlated with methanol or oleic acid induced proliferation of peroxisomes
    Skoneczny, M
    Swiezewska, E
    Szkopinska, A
    [J]. FEBS JOURNAL, 2005, 272 : 242 - 242
  • [2] ISOPRENOID BIOSYNTHESIS IN RAT-LIVER PEROXISOMES - CHARACTERIZATION OF CIS-PRENYLTRANSFERASE AND SQUALENE SYNTHETASE
    ERICSSON, J
    APPELKVIST, EL
    THELIN, A
    CHOJNACKI, T
    DALLNER, G
    [J]. JOURNAL OF BIOLOGICAL CHEMISTRY, 1992, 267 (26) : 18708 - 18714
  • [3] Products of S-cerevisiae cis-prenyltransferase activity in vitro
    Szkopinska, A
    Karst, F
    Palamarczyk, G
    [J]. BIOCHIMIE, 1996, 78 (02) : 111 - 116
  • [4] A conserved C-terminal RXG motif in the NgBR subunit of cis-prenyltransferase is critical for prenyltransferase activity
    Grabinska, Kariona A.
    Edani, Ban H.
    Park, Eon Joo
    Kraehling, Jan R.
    Sessa, William C.
    [J]. JOURNAL OF BIOLOGICAL CHEMISTRY, 2017, 292 (42) : 17351 - 17361
  • [5] Molecular Studies of the Protein Complexes Involving Cis-Prenyltransferase in Guayule (Parthenium argentatum), an Alternative Rubber-Producing Plant
    Lakusta, Adam M.
    Kwon, Moonhyuk
    Kwon, Eun-Joo G.
    Stonebloom, Solomon
    Scheller, Henrik, V
    Ro, Dae-Kyun
    [J]. FRONTIERS IN PLANT SCIENCE, 2019, 10
  • [6] Long-term effect of cyclic AMP on N-glycosylation is caused by an increase in the activity of the cis-prenyltransferase
    Konrad, M
    Merz, WE
    [J]. BIOCHEMICAL JOURNAL, 1996, 316 : 575 - 581
  • [7] Expression of Saccharomyces cerevisiae RER2 Gene Encoding Cis-Prenyltransferase in Trichoderma atroviride Increases the Activity of Secretory Hydrolases and Enhances Antimicrobial Features
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    Graczyk, Sebastian
    Pilsyk, Sebastian
    Lenart, Jacek
    Lipko, Agata
    Swiezewska, Ewa
    Bernat, Przemyslaw
    Kruszewska, Joanna S. S.
    [J]. JOURNAL OF FUNGI, 2023, 9 (01)
  • [8] Crystal structure of Thermobifida fusca cis-prenyltransferase reveals the dynamic nature of its RXG motif-mediated inter-subunit interactions critical for its catalytic activity
    Kurokawa, Hirofumi
    Ambo, Takanori
    Takahashi, Seiji
    Koyama, Tanetoshi
    [J]. BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS, 2020, 532 (03) : 459 - 465
  • [9] Protein glycosylation in pmt mutants of Saccharomyces cerevisiae.: Influence of heterologously expressed-cellobiohydrolase II of Trichoderma reesei and elevated levels of GDP-mannose and cis-prenyltransferase activity
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    Bankowska, Renata
    Palamarczyk, Grazyna
    Krotkiewski, Hubert
    Kruszewska, Joanna S.
    [J]. BIOCHIMICA ET BIOPHYSICA ACTA-GENERAL SUBJECTS, 2007, 1770 (05): : 774 - 780
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    Jin, Feng
    Zhang, Liwen
    Chen, Zhengxian
    Liu, Liang
    Parnell, Laurence D.
    Lai, Chao-Qiang
    Li, Duo
    [J]. JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY, 2010, 58 (16) : 9190 - 9197
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