Molecular cloning of a cDNA encoding an antigen which is salt-stably attached to centrosomes

被引：2
|
作者：
Angiolillo, A
Batova, I
Joswig, G
Werner, D
机构：
[1] UNIV PERUGIA,INST CELL & MOL BIOL,I-06123 PERUGIA,ITALY
[2] BULGARIAN ACAD SCI,INST BIOL & IMMUNOL,BU-1113 SOFIA,BULGARIA
[3] GERMAN CANC RES CTR,DIV CELLULAR BIOCHEM 0225,D-69009 HEIDELBERG,GERMANY
来源：
BIOCHIMICA ET BIOPHYSICA ACTA-GENE STRUCTURE AND EXPRESSION | 1996年 / 1309卷 / 03期
关键词：
cDNA expression-cloning; centrosome; centrosomal protein; coiled-coil; proline-rich region;
D O I：
10.1016/S0167-4781(96)00169-8
中图分类号：
Q5 [生物化学]; Q7 [分子生物学];
学科分类号：
071010 ; 081704 ;
摘要：
A monoclonal antibody (MAB 2A8) was used for expression-cloning of a complete cDNA (1133/5) to a mRNA (3 kb) encoding a murine 76 kDa polypeptide. The N-terminal section of the polypeptide is composed of domains capable to form alpha-helical coiled-coils. Its C-terminus is proline-rich and has characteristics of the Src homology region 3 (SH3). Affinity-purified antibodies to a recombinant section of the protein show that the antigen is salt-stably associated with the centrosome throughout the cell cycle.
引用
收藏
页码：194 / 196
页数：3
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