Peptide-Based SAMs that Resist the Adsorption of Proteins

被引：106

作者：

Chelmowski, Rolf ^{[1
]}

Koester, Stephan David ^{[1
]}

Kerstan, Andreas ^{[1
]}

Prekelt, Andreas ^{[1
]}

Grunwald, Christian ^{[2
]}

Winkler, Tobias ^{[3
]}

Metzler-Nolte, Nils ^{[1
]}

Terfort, Andreas ^{[3
]}

Woell, Christof ^{[1
]}

机构：

[1] Ruhr Univ Bochum, D-44780 Bochum, Germany

[2] Goethe Univ Frankfurt, Biochem Bioctr, D-60325 Frankfurt, Germany

[3] Univ Marburg, D-35032 Marburg, Germany

来源：

JOURNAL OF THE AMERICAN CHEMICAL SOCIETY | 2008年 / 130卷 / 45期

关键词：

D O I：

10.1021/ja8065754

中图分类号：

O6 [化学];

学科分类号：

0703 ;

摘要：

In this paper we present a modular approach for the fabrication of surfaces to characterize protein-protein interactions. The approach is based on azido peptides with an optimized sequence which are then thick functionalized using an alkynyl thiol and "click" chemistry. From these peptide thiols we fabricated SAMs on gold to evaluate the protein resistance, using surface plasmon resonance spectroscopy, toward streptavidin, bovin serum albumin (BSA), and fibronectin.

引用

页码：14952 / +

页数：3

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