Callus induction and regeneration in seed explants of rice (Oryza sativa CV. Swat-II)

Seeds of rice (Oryza sativa cv. Swat-II) were inoculated onto MS medium containing various concentrations of auxins and cytokinins. Excellent callus resulted on MS containing 2,4-D and Kn. However, this callus was non-embryogenic. Addition of tryptophan (TPN) @ 50 mg/l induced embryogenic callus. Increase in callus mass was obtained by a change in auxin and cytokinin level. Plantlet regeneration occurred oil MS containing BAP at 0.5 mg/l in combination with IAA at a concentration of 0.2 mg/l. The plantlets were transferred to the field after hardening.