Separation of glycomacropeptide (a functional protein material) by aqueous two-phase systems

被引：1
|
作者：
Wu, Jiang [1 ]
Wang, Yu [1 ]
Huang, Liang [1 ]
机构：
[1] Tianjin Agr Univ, Dept Agron, Tianjin 300384, Peoples R China
来源：
FUNCTIONAL MATERIALS AND NANOTECHNOLOGY | 2012年 / 496卷
关键词：
Aqueous two-phase systems; Extraction; PEG; Ammonium sulfate; Glycomacropeptide; BIOLOGICAL PRODUCTS; PARTITION; RECOVERY;
D O I：
10.4028/www.scientific.net/AMR.496.185
中图分类号：
TB3 [工程材料学];
学科分类号：
0805 ; 080502 ;
摘要：
PEG/ ammonium sulfate aqueous two-phase systems were supplied to separate GMP. It was found that PEG molecular weight, PEG concentration and ammonium sulfate concentration could influence the partitioning of GMP. The data of experiment showed that 18% (w/w) PEG6000 and 15% (w/w) ammonium sulfate were the optimum system, in which the protein recovery was 69.2%.
引用
收藏
页码：185 / 188
页数：4
相关论文
共 50 条
  • [1] Partitioning of glycomacropeptide in aqueous two-phase systems
    da Silva, C. A. S.
    Coimbra, J. S. R.
    Rojas, E. E. G.
    Teixeira, J. A. C.
    [J]. PROCESS BIOCHEMISTRY, 2009, 44 (11) : 1213 - 1216
  • [2] Separation of casein phosphopeptides (a functional food material) by aqueous two-phase systems
    Wu, Jiang
    Tong, Yingkai
    Ban, Litong
    [J]. ADVANCES IN CHEMISTRY RESEARCH II, PTS 1-3, 2012, 554-556 : 1511 - 1514
  • [3] Aqueous two-phase systems for protein separation: Phase separation and applications
    Asenjo, Juan A.
    Andrews, Barbara A.
    [J]. JOURNAL OF CHROMATOGRAPHY A, 2012, 1238 : 1 - 10
  • [4] Aqueous two-phase systems for protein separation: A perspective
    Asenjo, Juan A.
    Andrews, Barbara A.
    [J]. JOURNAL OF CHROMATOGRAPHY A, 2011, 1218 (49) : 8826 - 8835
  • [5] Aqueous Micellar Two-Phase Systems for Protein Separation
    Hirofumi Tani
    Tamio Kamidate
    Hiroto Watanabe
    [J]. Analytical Sciences, 1998, 14 : 875 - 888
  • [6] Aqueous micellar two-phase systems for protein separation
    Tani, H
    Kamidate, T
    Watanabe, H
    [J]. ANALYTICAL SCIENCES, 1998, 14 (05) : 875 - 888
  • [7] Aqueous two-phase systems for protein separation Studies on phase inversion
    Merchuk, JC
    Andrews, BA
    Asenjo, JA
    [J]. JOURNAL OF CHROMATOGRAPHY B, 1998, 711 (1-2): : 285 - 293
  • [8] NANOPARTICLE MEDIATED PROTEIN SEPARATION IN AQUEOUS MICELLAR TWO-PHASE SYSTEMS
    Fischer, Ingo
    Franzreb, Matthias
    [J]. SOLVENT EXTRACTION AND ION EXCHANGE, 2012, 30 (01) : 1 - 16
  • [9] Protein separation with magnetic adsorbents in micellar aqueous two-phase systems
    Becker, J. S.
    Thomas, O. R. T.
    Franzreb, M.
    [J]. SEPARATION AND PURIFICATION TECHNOLOGY, 2009, 65 (01) : 46 - 53
  • [10] Mechanism of Phase Separation in Aqueous Two-Phase Systems
    Titus, Amber R.
    Madeira, Pedro P.
    Ferreira, Luisa A.
    Chernyak, Vladimir Y.
    Uversky, Vladimir N.
    Zaslavsky, Boris Y.
    [J]. INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES, 2022, 23 (22)
12345