Cryo-electron microscopy structure of yeast Ty retrotransposon virus-like particles

被引：21
|
作者：
Palmer, KJ
Tichelaar, W
Myers, N
Burns, NR
Butcher, SJ
Kingsman, AJ
Fuller, SD
Saibil, HR
机构：
[1] UNIV LONDON BIRKBECK COLL, DEPT CRYSTALLOG, LONDON WC1E 7HX, ENGLAND
[2] BRITISH BIOTECH PHARMACEUT LTD, OXFORD, ENGLAND
[3] UNIV OXFORD, DEPT BIOCHEM, OXFORD OX1 3QU, ENGLAND
[4] EUROPEAN MOL BIOL LAB, D-69012 HEIDELBERG, GERMANY
来源：
JOURNAL OF VIROLOGY | 1997年 / 71卷 / 09期
关键词：
D O I：
10.1128/JVI.71.9.6863-6868.1997
中图分类号：
Q93 [微生物学];
学科分类号：
071005 ; 100705 ;
摘要：
The virus like particles (VLPs) produced by the yeast retrotransposon Ty1 are functionally related to retroviral cores. These particles are unusual in that they have variable radii. A paired mass-radius analysis of VLPs by scanning transmission electron microscopy showed that many of these particles farm an icosahedral T-number series. Three-dimensional reconstruction to 38-Angstrom resolution from cryo-electron micrographs of T=3 and T=4 shells revealed that the single structural protein encoded by the TYA gene assembles into spiky shells from trimeric units.
引用
收藏
页码：6863 / 6868
页数：6
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