Identification of weakly beta-hemolytic porcine spirochetes by biochemical reactions, PCR-based restriction fragment length polymorphism analysis and species-specific PCR

被引：3

作者：

Ohya, Tatsuo ^{[1
]}

Araki, Hiroshi ^{[2
]}

Sueyoshi, Masuo ^{[3
]}

机构：

[1] Natl Inst Anim Hlth, Environm Enzoot Dis Res Team, Kyushu Res Stn, Kagoshima 8910105, Japan

[2] Nagasaki Prefecture Kenhoku Livestock Hyg Serv Ct, Sasebo 858091, Japan

[3] Miyazaki Univ, Fac Agr, Dept Vet Hyg, Miyazaki 8892155, Japan

来源：

JOURNAL OF VETERINARY MEDICAL SCIENCE | 2008年 / 70卷 / 08期

关键词：

Brachyspira; PCR-RFLP; spirochete;

D O I：

10.1292/jvms.70.837

中图分类号：

S85 [动物医学（兽医学）];

学科分类号：

0906 ;

摘要：

We examined the usefulness of PCR-based restriction fragment length polymorphism (PCR-RFLP) and species-specific PCR combined with a newly devised rapid biochemical test using microplates for identifying weakly beta-hemolytic intestinal spirochetes (WBHIS) isolated from pigs. WBHIS strains showing atypical biochemical characteristics were decisively identified at the species level by PCR-RFLP and species-specific PCR. Identification of WBHIS at the species level in routine diagnostic work will certainly contribute to clarifying the pathogenicity of WBHIS.

引用

页码：837 / 840

页数：4

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