Fluorosis Caused Cellular Apoptosis and Oxidative Stress of Rat Kidneys

被引:7
|
作者
Song Yang [1 ]
Wang Jin-cheng [1 ]
Xu Hui [3 ]
Du Zhen-wu [2 ]
Zhang Gui-zhen [2 ]
Selim, Hamid Abdu [2 ]
Li Guang-sheng [3 ]
Wang Qing [2 ]
Gao Zhong-li [1 ]
机构
[1] Jilin Univ, China Japan Union Hosp, Dept Orthoped, Changchun 130033, Peoples R China
[2] Jilin Univ, China Japan Union Hosp, Cent Lab, Changchun 130033, Peoples R China
[3] Jilin Univ, Inst Endem Dis, Pathol Lab, Changchun 130021, Peoples R China
基金
中国国家自然科学基金;
关键词
Fluorosis; Apoptosis; Oxidative stress; FLUORIDE; DAMAGE;
D O I
10.1007/s40242-013-2430-2
中图分类号
O6 [化学];
学科分类号
0703 ;
摘要
As the strongest electronegative element, fluorine can stimulate the production of superoxide radicals in cells. In view of the important roles of kidneys in bone metabolism, the authors analyzed the quantitative pathomorphological characteristics of renal damage and the potential cellular apoptosis and oxidative stress mechanisms in rats treated with excessive fluoride. Wistar rats were exposed to 50 mg F-(110.5 mg NaF)/L, 100 mg F-(221.0 mg NaF)/L and 150 mg F-(331.5 mg NaF)/L in drinking water for 70 and 140 d, respectively. Microscope with image analysis was used to quantitate pathomorphological changes in renal tissues of the rats. Reactive oxygen species(ROS), the cell cycle and apoptosis of renal cells were measured by flow cytometry and TUNEL technique(terminal deoxynucleotidyl transferase dUTP nick end labeling), respectively. The ion concentrations in serum and renal functional parameters were detected by automatic biochemical analyzer. Quantitative analysis results demonstrate the expanded Bowman's space of glomerulus and obvious dilatation of renal tubule. TUNEL technique revealed that NBT/BCIP (nitro blue tetrazolium/5-bromo-4-chloro-3'-indolylphosphate, p-toluidine salt)-staining positive apoptotic cells selectively located in medullocortical junction areas. The data suggest that renal damage in chronic fluorostic rats is associated with the cellular apoptosis and oxidative stress.
引用
收藏
页码:263 / 269
页数:7
相关论文
共 50 条
  • [1] Fluorosis caused cellular apoptosis and oxidative stress of rat kidneys
    Yang Song
    Jin-cheng Wang
    Hui Xu
    Zhen-wu Du
    Gui-zhen Zhang
    Hamid Abdu Selim
    Guang-sheng Li
    Qing Wang
    Zhong-li Gao
    Chemical Research in Chinese Universities, 2013, 29 : 263 - 269
  • [2] Evaluation of oxidative stress during apoptosis and necrosis caused by carbon tetrachloride in rat liver
    Sun, F
    Hamagawa, E
    Tsutsui, C
    Ono, Y
    Ogiri, Y
    Kojo, S
    BIOCHIMICA ET BIOPHYSICA ACTA-MOLECULAR BASIS OF DISEASE, 2001, 1535 (02): : 186 - 191
  • [3] Long-term exposure to copper induces autophagy and apoptosis through oxidative stress in rat kidneys
    Wan, Fang
    Zhong, Gaolong
    Ning, Zhijun
    Liao, Jianzhao
    Yu, Wenlan
    Wang, Congcong
    Han, Qingyue
    Li, Ying
    Pan, Jiaqiang
    Tang, Zhaoxin
    Huang, Riming
    Hu, Lianmei
    ECOTOXICOLOGY AND ENVIRONMENTAL SAFETY, 2020, 190
  • [4] Effect of DHA plus EPA on oxidative stress and apoptosis induced by ischemia-reperfusion in rat kidneys
    Ajami, Marjan
    Davoodi, Sayed Hossein
    Habibey, Rouhollah
    Namazi, Nasim
    Soleimani, Mansoureh
    Pazoki-Toroudi, Hamidreza
    FUNDAMENTAL & CLINICAL PHARMACOLOGY, 2013, 27 (06) : 593 - 602
  • [5] Propofol ameliorates doxorubicin-induced oxidative stress and cellular apoptosis in rat cardiomyocytes
    Lai, H. C.
    Yeh, Y. C.
    Wang, L. C.
    Ting, C. T.
    Lee, W. L.
    Lee, H. W.
    Wang, K. Y.
    Wu, A.
    Su, C. S.
    Liu, T. J.
    TOXICOLOGY AND APPLIED PHARMACOLOGY, 2011, 257 (03) : 437 - 448
  • [6] Evaluation of oxidative stress during apoptosis and necrosis caused by D-galactosamine in rat liver
    Sun, F
    Hamagawa, E
    Tsutsui, C
    Sakaguchi, N
    Kakuta, Y
    Tokumaru, S
    Kojo, S
    BIOCHEMICAL PHARMACOLOGY, 2003, 65 (01) : 101 - 107
  • [7] Effects of propofol and dexedetomidin on oxidative stress in rat lung and kidneys
    Taslipinar, M. Yavuz
    Urfalioglu, A.
    Akcaboy, E. Y.
    Bayraktar, N.
    Kilic, N.
    FEBS JOURNAL, 2006, 273 : 183 - 183
  • [8] Micromeria congesta Alleviates LPS-Induced Inflammation, Apoptosis, Oxidative Stress and DNA Damage in Rat Heart and Kidneys
    Dortbudak, Muhammet Bahaeddin
    Demircioglu, Muhammed
    Kapucuk, Fuat Serkan
    VETERINARY MEDICINE AND SCIENCE, 2025, 11 (02)
  • [9] The cardioprotective effects of L-carnitine on rat cardiac injury, apoptosis, and oxidative stress caused by amethopterin
    Ehab Tousson
    Ezar Hafez
    Somia Zaki
    Amani Gad
    Environmental Science and Pollution Research, 2016, 23 : 20600 - 20608
  • [10] The cardioprotective effects of L-carnitine on rat cardiac injury, apoptosis, and oxidative stress caused by amethopterin
    Tousson, Ehab
    Hafez, Ezar
    Zaki, Somia
    Gad, Amani
    ENVIRONMENTAL SCIENCE AND POLLUTION RESEARCH, 2016, 23 (20) : 20600 - 20608