Wound healing by a 3.2 kDa recombinant polypeptide from velvet antler of Cervus nippon Temminck

被引：21

作者：

Zha, Enhui ^{[1
,2
]}

Gao, Shenyang ^{[3
]}

Pi, Yuzhen ^{[1
]}

Li, Xingxia ^{[4
]}

Wang, Yutain ^{[2
]}

Yue, Xiqing ^{[1
]}

机构：

[1] Shenyang Agr Univ, Coll Food Sci, Shenyang 110866, Peoples R China

[2] Liaoning Med Univ, Inst Food Sci & Engn, Jinzhou 121001, Peoples R China

[3] Liaoning Med Univ, Coll Anim Husb & Vet Med, Jinzhou 121001, Peoples R China

[4] Yantai Engn & Technol Coll, Yantai 264006, Peoples R China

来源：

BIOTECHNOLOGY LETTERS | 2012年 / 34卷 / 04期

关键词：

NIH3T3; cells; Recombinant polypeptide; Velvet antler polypeptide; Wound healing; GROWTH-FACTOR; PROLIFERATION; ELAPHUS; EXTRACT;

D O I：

10.1007/s10529-011-0829-8

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

Velvet antler (VA) is used in traditional Chinese medicine to treat a wide range of ailments including the enhancement of wound healing. A 3.2 kDa recombinant polypeptide of VA from sika deer was purified and compared to native polypeptides stimulation growth of NIH3T3 cells. Both stimulated growth in a dose-dependent manner (10-100 mu g/ml). To study its wound healing properties, burn-wounded rats were topically administered with recombinant VA polypeptide or native polypeptide. Rats treated with 0.05 and 0.1% (w/w) polypeptides exhibited significant wound healing. As the yield of recombinant polypeptide was 40-fold higher than that of the native polypeptide, it may therefore be a useful biopharmaceutical.

引用

页码：789 / 793

页数：5

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