Effects of megagametophyte removal on DNA yield and early seedling growth in coastal Douglas-fir

被引：3
|
作者：
Krutovskii, KV
Vollmer, SS
Sorensen, FC
Adams, WT
Strauss, SH
机构：
[1] OREGON STATE UNIV, DEPT FOREST SCI, CORVALLIS, OR 97331 USA
[2] US FOREST SERV, USDA, FORESTRY SCI LAB, CORVALLIS, OR 97331 USA
[3] NI VAVILOV GEN GENET RES INST, LAB POPULAT GENET, MOSCOW 117809, RUSSIA
来源：
CANADIAN JOURNAL OF FOREST RESEARCH | 1997年 / 27卷 / 06期
关键词：
D O I：
10.1139/cjfr-27-6-964
中图分类号：
S7 [林业];
学科分类号：
0829 ; 0907 ;
摘要：
Stratified seeds of coastal Douglas-fir (Pseudotsuga menziesii (Mirb.) Franco var, menziesii) were germinated, sown in soil, and seed coats and megagametophytes were removed at various stages of early seedling development. Yield and quality of DNA extracted from the megagametophytes were related to several morphological trails of the seedlings after 2 months of growth in a controlled environment chamber. Regression and analysis of variance demonstrated nonlinear associations between stage of megagamelophyte removal and seedling size traits, DNA yield and quality, and RNA presence. Megagametophyte removal when cotyledons had extended one-quarter of their length (about 4 mm) outside the seed coat (our stage 4) resulted in sufficient DNA for construction of saturated PCR-(polymerase chain reaction) based genome maps and had little effect on seedling development.
引用
收藏
页码：964 / 968
页数：5
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