Examining genetic relationships of Chinese Pleurotus ostreatus cultivars by combined RAPD and SRAP markers

被引：11
|
作者：
Yin, Yonggang [1 ,2 ]
Liu, Yu [1 ]
Wang, Shouxian [1 ]
Zhao, Shuang [1 ]
Xu, Feng [1 ]
机构：
[1] Beijing Acad Agr & Forestry Sci, Inst Plant & Environm Protect, Beijing 100097, Peoples R China
[2] Agr Univ Hebei, Coll Hort, Baoding 071001, Hebei, Peoples R China
来源：
MYCOSCIENCE | 2013年 / 54卷 / 03期
关键词：
Genetic diversity; Molecular marker; Polymorphism; UPGMA; DIVERSITY;
D O I：
10.1016/j.myc.2012.09.015
中图分类号：
Q93 [微生物学];
学科分类号：
071005 ; 100705 ;
摘要：
Combined randomly amplified polymorphic DNA (RAPD) and sequence-related amplified polymorphism (SRAP) were used to assess the genetic diversity of Pleurotus ostreatus strains cultivated in China. For the RAPD and SPAP analyses, 479 and 282 polymorphic bands were obtained from 20 P. ostreatus strains using 20 and 13 selected primers or primer pairs, respectively. A combined RAPD/SRAP dendrogram grouped the 20 strains into five clades with a coefficient of 0.690. The comparison of RAPD and SRAP was evaluated in the present study. The combined RAPD/SRAP markers provided reliable information regarding the relationships among the P. ostreatus strains. (C) 2012 The Mycological Society of Japan. Published by Elsevier B.V. All rights reserved.
引用
收藏
页码：221 / 225
页数：5
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