Evaluation of a gel-permeation high-performance liquid chromatography for determining triglyceride levels in serum major lipoproteins, compared with the ultracentrifugation/precipitation method

Objectives: To evaluate the gel permeation high-performance liquid chromatography (GP-HPLC) method for determination of triglyceride (TG) levels in low-density lipoprotein (LDL) and high-density lipoprotein (HDL). Design and methods: The GP-HPLC and the ultracentrifugation(UC)/precipitation methods were used and compared. Results: There was no significant difference in measured levels of LDL-triglyceride between the two methods, but the HDL-triglyceride levels measured by the GP-HPLC technique were significantly higher than the UC/precipitation one (145 +/- 47 mg/L and 121 +/- 45 mg/L respectively, n=38, p<0.0001). Conclusions: A GP-HPLC technique provides LDL-TG levels comparable to those obtained by the UC/precipitation method. (C) 2008 The Canadian Society of Clinical Chemists. Published by Elsevier Inc. All rights reserved.