Circular dichroism spectroscopy reveals invariant conformation of guanine runs in DNA

被引:99
|
作者
Kypr, J [1 ]
Vorlícková, M [1 ]
机构
[1] Acad Sci Czech Republ, Inst Biophys, CZ-61265 Brno, Czech Republic
关键词
circular dichroism; spectroscopy; DNA; guanine; tetraplex; single strand;
D O I
10.1002/bip.10112
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
We demonstrate that the characteristic circular dichroism (CD) features of the parallel-stranded DNA tetraplex of d(G(4)), especially the strong band at 260 nm, are characteristic for the B and A forms of the antiparallel duplex of d(C(4)G(4)). Hence, this band evidently originates from intrastrand guanine-guanine stacking, which is therefore very similar in the duplex and tetraplex DNA. In addition, the same type of the CD spectrum is provided by the ordered single strand of d(GA)(10). This observation suggests that the ordered single strand of d(GA)(10) is stabilized by a core of guanines stacked like in the parallel tetraplex. This view is used to start the modeling of the molecular structure of the, ordered d(GA)(10) single strand. Our studies suggest that guanine itself is strong enough to stabilize various secondary structures of DNA, which is a property relevant to thinking about the origin and evolution of molecular replicators. (C) 2002 Wiley Periodicals, Inc.
引用
收藏
页码:275 / 277
页数:3
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