Isolation and characterization of a Δ5-desaturase from Oblongichytrium sp.

被引：10

作者：

Kumon, Yasuyuki ^{[1
]}

Kamisaka, Yasushi ^{[1
]}

Tomita, Nao ^{[1
]}

Kimura, Kazuyoshi ^{[1
]}

Uemura, Hiroshi ^{[1
]}

Yokochi, Toshihiro ^{[1
]}

Yokoyama, Rinka ^{[2
]}

Honda, Daiske ^{[2
]}

机构：

[1] Natl Inst Adv Ind Sci & Technol, Inst Biol Resources & Funct, Tsukuba, Ibaraki 3058566, Japan

[2] Konan Univ, Dept Biol, Fac Sci, Kobe, Hyogo 6588501, Japan

来源：

BIOSCIENCE BIOTECHNOLOGY AND BIOCHEMISTRY | 2008年 / 72卷 / 08期

关键词：

Delta; 5-desaturase; Labyrinthulomycetes; Oblongichytrium; polyunsaturated fatty acids; thraustochytrid;

D O I：

10.1271/bbb.80235

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

We isolated a cDNA clone with homology to known desaturase genes from Oblongichytrium sp., recently classified as a new genus of thraustochytrids (Labyrinthulomycetes), and found that it encoded Delta 5-desaturase by its heterologous expression in yeast. The enzyme had higher activity toward 20:4n-3 than 20:3n-6, indicating that this Delta 5-desaturase can be used in the production of n-3 polyunsaturated fatty acids in transgenic organisms.

引用

页码：2224 / 2227

页数：4

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