Use of biometric embryonic growth parameters as indicator of exposure to a teratogen

被引:0
|
作者
Amwayi, PJA
OtiangAOwti, GE
机构
关键词
D O I
暂无
中图分类号
R5 [内科学];
学科分类号
1002 ; 100201 ;
摘要
Disturbances in embryonic grow-th were studied in 233 foetuses harvested on day 14.5 of gestation, after the administration of various doses of 5 Fluoro-2' deoxyuridine (FUdR) to pregnant mice on day 11.0 of gestation, Measurements of crown-rump length (CRL) and mean wet body weights showed a significant retardation of embryonic growth (p<0.001), following doses of 30, 80 and 100 mg FUdR per kg maternal body weight, Compared to the controls, whole FUdR-treated embryos that had been macerated, cleared and double shined with alcian blue 8GX plus alizarin red S for skeletal anlage, showed that ossification had not commenced in the vertebral bones of tail, All bones in the craniofacial region and limbs including the girdles, were smaller, while there were distortions of the long bones, The severity of the changes were dependent on the concentration of FUdR dose administered, Among the live FUdR-treated foetuses harvested, 95% had mesomelic limb defects, The incidence of delay or prevention of palatal processes elevation was 79%, 49%, 21%, and 30% respectively for 0 mg (control), 30 mg, 80 mg and 100 mg FUdR doses, The results show that administration of a teratogenic agent (FUdR) causes retardation of growth which correlates with abnormalities of the secondary palate and limbs, It is proposed that the initial screening of potential teratogenic substances in food, such as preservatives or colourings, may be carried out by monitoring changes in secondary palate and limb development, including biometric growth parameters of an animal model.
引用
收藏
页码:6 / 11
页数:6
相关论文
共 50 条
  • [1] EMBRYONIC RECOVERY AFTER EXPOSURE TO A TERATOGEN
    SHUM, L
    SADLER, TW
    TERATOLOGY, 1988, 37 (05) : 492 - 492
  • [2] SOME BIOMETRIC PROBLEMS OF EMBRYONIC GROWTH
    ELIAS, S
    TERATOLOGY, 1973, 8 (02) : 219 - 219
  • [3] Birth Defects and Neonatal Morbidity Caused by Teratogen Exposure After the Embryonic Period
    Scheuerle, Angela E.
    Aylsworth, Arthur S.
    BIRTH DEFECTS RESEARCH PART A-CLINICAL AND MOLECULAR TERATOLOGY, 2016, 106 (11) : 935 - 939
  • [4] Pregnancy outcome following exposure to permethrin and use of teratogen information
    Kennedy, D
    Hurst, V
    Konradsdottir, E
    Einarson, A
    AMERICAN JOURNAL OF PERINATOLOGY, 2005, 22 (02) : 87 - 90
  • [5] Fetal Growth Restriction: Comparison of Biometric Parameters
    Marchand, Carolin
    Koeppe, Jeanette
    Koester, Helen Ann
    Oelmeier, Kathrin
    Schmitz, Ralf
    Steinhard, Johannes
    Fruscalzo, Arrigo
    Kubiak, Karol
    JOURNAL OF PERSONALIZED MEDICINE, 2022, 12 (07):
  • [6] Distribution of ocular biometric parameters during the eye growth
    Yekta, A.
    Hashemi, H.
    Ostadimoghaddam, H.
    Jafarzadehpour, E.
    Khabazkhoob, M.
    Rezvan, F.
    ACTA OPHTHALMOLOGICA, 2013, 91
  • [7] GROWTH RETARDATION AND CELL-DEATH IN MOUSE EMBRYOS FOLLOWING EXPOSURE TO TERATOGEN BROMODEOXYURIDINE
    PACKARD, DS
    SKALKO, RG
    MENZIES, RA
    EXPERIMENTAL AND MOLECULAR PATHOLOGY, 1974, 21 (03) : 351 - 362
  • [8] BIOMETRIC PARAMETERS OF PLANTING MATERIAL AS A TEST INDICATOR OF SUCCESS OF Pinus silvestris L PLANTATIONS
    Sungurova, Natalia R.
    Drochkova, Anna A.
    LESNOY ZHURNAL-FORESTRY JOURNAL, 2021, (04) : 107 - 116
  • [9] Embryonic trout growth and boron exposure.
    Eckhert, C
    FASEB JOURNAL, 1997, 11 (03): : 2350 - 2350
  • [10] Functional data analysis in the use of eyebrow shape as a biometric indicator in face recognition
    Albert, A. Midori
    Chen, Cuixian
    Wang, Yishi
    Chang, Yaw
    INTERNATIONAL JOURNAL OF BIOMETRICS, 2014, 6 (02) : 166 - 179