Effect of light on the organogenic ability of garlic roots using a one-step in vitro system

被引：11
|
作者：
Martín-Urdíroz, N [1 ]
Garrido-Gala, J [1 ]
Martín, J [1 ]
Barandiaran, X [1 ]
机构：
[1] Univ Cordoba, Fac Ciencias, Dept Microbiol, Cordoba 14071, Spain
来源：
PLANT CELL REPORTS | 2004年 / 22卷 / 10期
关键词：
allium sativum; genetic variability; light effects; regeneration system;
D O I：
10.1007/s00299-003-0744-0
中图分类号：
Q94 [植物学];
学科分类号：
071001 ;
摘要：
A simple and efficient garlic in vitro shoot regeneration protocol has been developed. This system uses axenic root tips cultivated from the beginning in the presence of light and does not require any change or refreshing of the original medium during the entire process. The application of light from the beginning of the culture process did not affect the callus formation rate but did significantly improve the explant regeneration ability. In a 2-month period it was possible to obtain up to 250 shoots per gram of callus.
引用
收藏
页码：721 / 724
页数：4
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