The immunoreactivity and activity of adenylate cyclase type I are changed in the hippocampal CA1 region after transient forebrain ischemia in gerbils

Adenylate cyclase (AC) has a specific sensitivity to Ca2+/calmodulin. AC-1. one of the mediator of learning and memory, plays an important role in signal transduction underlying learning and memory function. In the present study, we found ischemia-related changes of AC-1 in the hippocampal CA1 region, but not in the CA2/3 region, after 5 min of transient forebrain ischemia in gerbils. In the sham-operated group, AC-1 immunoreactive neurons were detected in pyramidal and non-pyramidal cells in the hippocampus proper. AC-1 immunoreactivity was significantly increased at 3 h in the CA1 region after ischemic insult. Thereafter, AC-I immunoreactivity was gradually decreased. Four days after ischemic insult, AC-I-immunoreactive CA1 pyramidal cells in the stratum pyramidale were very few due to delayed neuronal death. The results of Western blot analysis showed that changes of AC-1 protein contents were similar to immunohistochemical data after ischemic insult. Gpp(NH)p-dependent AC-1 activity in hippocampal CA1region was not changed in all groups, while Ca2+/calmodulin-dependent AC-1 activity in hippocarmpal CA1 region was significantly decreased 24 h after ischemia-reperfusion. These results suggest that the decrease of AC-1 activity may be associated with impairment of neurodevelopment and neuroplasticity including learning and memory although the AC-1 immunoreactivity was maintained 24 h postischemic group compared to that of the sham-operated group. (c) 2005 Elsevier B.V All rights reserved.