Simultaneous immunostaining with anti-S100P and anti-SV40 antibodies revealed the origin of BK virus-infected decoy cells in voided urine samples

被引：6

作者：

Ariyasu, S. ^{[1
,2
]}

Yanai, H. ^{[3
]}

Sato, M. ^{[4
]}

Shinno, Y. ^{[2
]}

Taniguchi, K. ^{[2
]}

Yamadori, I. ^{[5
]}

Miki, Y. ^{[6
]}

Sato, Y. ^{[1
]}

Yoshino, T. ^{[7
]}

Takahashi, K. ^{[1
]}

机构：

[1] Okayama Univ, Grad Sch Hlth Sci, Dept Med Technol, Okayama, Japan

[2] Okayama Med Ctr, Natl Hosp Org, Dept Clin Lab, Okayama, Okayama 7011192, Japan

[3] Okayama Univ Hosp, Dept Pathol, Okayama, Japan

[4] Shikoku Canc Ctr, Dept Clin Lab, Natl Hosp Org, Mastuyama, Japan

[5] Hiroshima City Hiroshima Citizens Hosp, Dept Pathol, Hiroshima, Japan

[6] Kochi Gakuen Coll, Dept Med Technol, Kochi, Japan

[7] Okayama Univ, Grad Sch Med Dent & Pharmaceut Sci, Dept Pathol, Okayama, Japan

来源：

CYTOPATHOLOGY | 2015年 / 26卷 / 04期

关键词：

BK virus-infected cells; S100P; SV40; simultaneous immunostaining; urine cytology; POLYOMAVIRUS-ASSOCIATED NEPHROPATHY; TRANSPLANT RECIPIENTS; RENAL-TRANSPLANTATION; UROTHELIAL CARCINOMA; CYTOLOGY; DIFFERENTIATION; ALLOGRAFTS; DIAGNOSIS; DISEASE; UPDATE;

D O I：

10.1111/cyt.12213

中图分类号：

Q2 [细胞生物学];

学科分类号：

071009 ; 090102 ;

摘要：

BackgroundMethods for determining the origin of BK virus (BKV)-infected cells (decoy cells) in clinical urine samples have not been established although they could enhance the diagnosis of BKV infection in immunocompromised patients. MethodsWe performed simultaneous immunostaining with anti-S100P (a urothelial marker) and anti-SV40 antibodies in 66 clinical urine samples exhibiting SV40 positivity and a decoy-cell appearance on Papanicolaou staining. The clinical voided urine samples included seven cases of renal transplantation, 47 cases of cancer therapy and 12 cases of non-neoplastic disease. SurePath liquid-based cytology was used for the urine samples. ResultsBKV-infected cells were categorized as SV40(+)/S100P(+) and SV40 (+)/S100p(-). SV40(+)/S100P(-) cells were found in 55 cases (83.4%); nine cases (13.6%) carried both SV40(+)/S100P(-) and SV40(+)/S100P(+) cells. The former were identified as BKV infection in renal tubules and the latter in both the renal tubules and urothelial epithelia. The remaining two cases (3.0%) had only SV40(+)/S100P(+) cells of urothelial origin. ConclusionSimultaneous immunostaining with anti-S100P and anti-SV40 is a useful method for determining the origin of BKV-infected cells in clinical urine samples from immunocompromised patients such as renal transplantation recipients.

引用

页码：250 / 255

页数：6