Identification of sex using SBNO1 gene in the Chinese softshell turtle, Pelodiscus sinensis (Trionychidae)

被引：4
|
作者：
Zhao, Lan [1 ,2 ]
Wang, Xin [1 ,2 ]
Wan, Qiu-Hong [1 ,2 ]
Fang, Sheng-Guo [1 ,2 ]
机构：
[1] Zhejiang Univ, Key Lab Conservat Biol Endangered Wildlife, Minist Educ, Coll Life Sci, Hangzhou 310058, Zhejiang, Peoples R China
[2] Zhejiang Univ, State Conservat Ctr Gene Resources Endangered Wil, Coll Life Sci, Hangzhou 310058, Zhejiang, Peoples R China
来源：
JOURNAL OF GENETICS | 2019年 / 98卷 / 02期
关键词：
polymerase chain reaction; sex identification; sex chromosome; molecular sexing; reptile; Chinese softshell turtle; CHROMOSOMES;
D O I：
10.1007/s12041-018-1048-z
中图分类号：
Q3 [遗传学];
学科分类号：
071007 ; 090102 ;
摘要：
The Chinese softshell turtle exhibits ZZ/ZW sex determination. To identify the sex of embryos, juvenile and adult individuals, we designed two pairs of polymerase chain reaction primers, SB1-196, which amplifies a fragment of 196 bp in the female and the other, CK1-482, which amplifies the 482-bp fragment in both the sexes. It is validated in 24 adult turtles of known sex, sampled from three different locations. This one-step sexing technique is rapid and easy to perform and is reported for the first time.
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页数：4
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