Identification of mosquito bloodmeals using mitochondrial cytochrome oxidase subunit I and cytochrome b gene sequences

被引：99

作者：

Townzen, J. S. ^{[1
]}

Brower, A. V. Z. ^{[2
]}

Judd, D. D. ^{[1
]}

机构：

[1] Oregon State Univ, Corvallis, OR 97331 USA

[2] Middle Tennessee State Univ, Dept Biol, Murfreesboro, TN 37130 USA

来源：

MEDICAL AND VETERINARY ENTOMOLOGY | 2008年 / 22卷 / 04期

关键词：

Bloodmeal analysis; cytochrome b; cytochrome oxidase subunit I; DNA barcode; GenBank; mosquitoes;

D O I：

10.1111/j.1365-2915.2008.00760.x

中图分类号：

Q96 [昆虫学];

学科分类号：

摘要：

Primer pairs were designed and protocols developed to selectively amplify segments of vertebrate mitochondrial cytochrome oxidase subunit 1 (COI) and cytochrome b (Cyt b) mtDNA from the bloodmeals of mosquitoes (Diptera: Culicidae). The protocols use two pairs of nested COI primers and one pair of Cyt b primers to amplify short segments of DNA. Resultant sequences are then compared with sequences in GenBank, using the BLAST function, for putative host identification. Vertebrate DNA was amplified from 88% of our sample of 162 wild-caught, blood-fed mosquitoes from Oregon, U.S.A. and GenBank BLAST searches putatively identified 98% of the amplified sequences, including one amphibian, seven mammalian and 14 avian species. Criteria and caveats for putative identification of bloodmeals are discussed.

引用

页码：386 / 393

页数：8

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