Fluorescence probe with a pH-sensitive trigger

被引：35

作者：

Galande, AK ^{[1
]}

Weissleder, R ^{[1
]}

Tung, CH ^{[1
]}

机构：

[1] Harvard Univ, Sch Med, Massachusetts Gen Hosp, Ctr Mol Imaging Res, Charlestown, MA 02129 USA

来源：

BIOCONJUGATE CHEMISTRY | 2006年 / 17卷 / 02期

关键词：

D O I：

10.1021/bc050330e

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

Acid-catalyzed hydrolysis was used as the mechanism to design a new type of environmentally sensitive fluorescence probe. A mild and selective periodate oxidation of the 2-amino alcohol of serine in the presence of a disulfide bond was developed to prepare dialdehyde peptides. Two identical fluorochrome hydrazide derivatives were then linked to the dialdehyde peptide forming an acid-labile hydrazone linkage. This self-quenched probe is weakly fluorescent at a physiological pH of 7.4 but shows more than 3-fold fluorescence enhancement at pH 4.5.

引用

页码：255 / 257

页数：3

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