Adsorptive transfer voltammetry applied to the study of chromium-induced DNA damage in the presence of curcumin

被引:3
|
作者
Serpi, Constantina [1 ]
Stanic, Zorka [2 ]
Girousi, Stella [1 ]
机构
[1] Aristotle Univ Thessaloniki, Analyt Chem Lab, Dept Chem, Thessaloniki 54124, Greece
[2] Univ Kragujevac, Dept Chem, Fac Sci, Kragujevac 34000, Serbia
关键词
DNA interaction; chromium(VI); curcumin; carbon paste electrode; voltammetry; ELECTROCHEMICAL BIOSENSOR; ELECTRODES; CU(II); ACID; GRAPHITE; TOXICITY; RADICALS; CHROMATE; INVITRO; PASTE;
D O I
10.1080/03067319.2012.656098
中图分类号
O65 [分析化学];
学科分类号
070302 ; 081704 ;
摘要
The interaction between double-stranded (ds) calf-thymus DNA and chromium in the presence of curcumin (CC) was studied by differential pulse adsorptive transfer voltammetry using carbon paste electrode (CPE). CurcuminCr complex generated changes in calf thymus DNA. The mechanism for DNA cleavage by curcuminCr complex appears to involve both the hydroxyl radical as well as singlet oxygen. The characteristic peak of dsDNA, due to the oxidation of guanine residues, drastically decreased. The increased DNA damage by curcuminCr complex was observed in the presence of various concentrations of chromium(VI).
引用
收藏
页码:543 / 552
页数:10
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