A role for the hinge/ear domain of the beta chains in the incorporation of AP complexes into clathrin-coated pits and coated vesicles

被引:14
|
作者
Clairmont, KB
Boll, W
Ericsson, M
Kirchhausen, T
机构
[1] HARVARD UNIV,SCH MED,DEPT CELL BIOL,BOSTON,MA 02115
[2] HARVARD UNIV,SCH MED,CTR BLOOD RES,BOSTON,MA 02115
关键词
coated pits; vesicles; endocytosis; adaptors; coat assembly;
D O I
10.1007/s000180050080
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
The clathrin-associated adaptor protein (AP) complexes drive the polymerization of clathrin in coated pits to form coated vesicles. It has previously been shown that the carboxyl-terminal hinge/ear domain of the beta 2 chain contains a binding site for clathrin and that removal of this domain from APs or from isolated beta 2 chains abrogates their ability to form clathrin coats in vitro. We show here that the hinge/ear domain is necessary for efficient incorporation of AP complexes into coated pits and coated vesicles in cells, a result that is consistent with the view that the beta chains indeed provide an important interaction between the AP complexes and clathrin.
引用
收藏
页码:611 / 619
页数:9
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