Catalytic and DNA-hydrolyzing activities of purified immunoglobulins IgG from patients sera with autoimmune disease by pseuclobiospecific chromatography using histicly-aminohexyl-sepharose

被引：6

作者：

Elkak, A

Bourhim, M

Coffinier, Y

Vijayalakshmi, MA

机构：

[1] Lebanese Univ, Fac Pharm, Dept Med Biol, Beirut 6573, Lebanon

[2] Natl Council Sci Res, Beirut, Lebanon

[3] Univ Technol Compiegne, Lab Interact Mol & Technol Separat, F-60205 Compiegne, France

来源：

CHROMATOGRAPHIA | 2004年 / 60卷 / 3-4期

关键词：

column liquid chromatography; pseudobioaffinity chromatography; histidine-aminokexyl-sepharose gel; autoimmune diseases; DNA-hydrolysis;

D O I：

10.1365/s10337-004-0333-6

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

Catalytic autoimmune antibodies from patients with antiphospholipid (aPL) antibodies were purified using histidyl-aminohexyl-sepharose gel. The sera were loaded on the columns equilibrated with 25 mM MOPS buffer pH 7.4 and the absorbed proteins were eluted by adding 0.2 M NaCl in the equilibrating buffer. Antibodies purity was evaluated by SDS-PAGE. The purified immunoglobulins G from patients with (aPL) sera by histidyl-aminohexyl-sepharose ;show DNA-degrading activity of the plasmid pUC19 DNA and catalytic activity in hydrolyzing the pepticle substrate Pro-Phe-Arg-7-amido-4-methylcoumarin.

引用

页码：165 / 169

页数：5