In vivo Evaluation of Microemulsion-Based Transdermal Gel of Coumarins from Cortex Daphnes

Objetive The aim of the present study was to investigate the pharmacokinetics of coumarins from cortex daphnes (CCD) in a microemulsion (ME) gel transdermal delivery system with a newly developed HPLC method. Methods The pharmacokinetics of the microemulsion containing CCD was studied in rabbits with the established HPLC method. Results The results showed that among the three index compounds of CCD, the daphnin and daphne-8-O-beta-D-glucoside from ME gel presented significant higher AUC((0-infinity)) and C-max compared to the conventional preparation (CP), while daphnetin showed similar AUC((0-infinity)) and higher Cmax compared to the latter. Conclusion Hence, the established HPLC method was suitable to analyze the CCD components in ME and the ME gel could be a promising transdermal delivery system for CCD.