Effect of Dimethyl Sulfoxide on Peripheral Blood-Originating Mesenchymal Progenitor Cells Viability

Background: Mesenchymal Progenitor Cells-Peripheral Blood (MPC-PB) are multipotent cells which acts as a precursor of connective tissues and has the potential for the regeneration of joints cartilage. This study focuses on MPCs originating from peripheral blood due to its less invasive nature for obtaining them compared to bone marrow aspiration. Me2SO (dimethyl sulfoxide) were used in cryopreservation and culture processes for preventing cell damage from low temperature. However, high concentrations of Me2SO may decrease cell viability. This study is aimed to determine the effect of Me2SO towards peripheral blood-originating MPCs. Methods: MPC-PB were placed in a 96-well plate with a density of 2600 cells per well. The experiments were divided to 9 treatments; control, Me2SO 0,5%, 1%, 1,5%, 2%, 2,5%, 3%, 3,5%, and 4%. The study was held for 15 days, and cell count were calculated using WST-1 reagent and microplate reader. Results: We found a cell viability difference between each treatment. MPCs exposed to Me2SO for 9 days with a Me2SO concentration of 0,5% dan 1% had a reversible change, while MPCs exposed to Me2SO above 1% had an irreversible change. Conclusion: The results of this study showed that certain Me2SO levels affect the viability of peripheral blood-originating in vitro-cultured MPCs.