The chromosome map of Bacillus thuringiensis subsp canadensis HD224 is highly similar to that of the Bacillus cereus type strain ATCC 14579

被引:0
|
作者
Carlson, CR
Johansen, T
Kolsto, AB
机构
[1] UNIV OSLO,INST PHARM,N-0316 OSLO,NORWAY
[2] UNIV OSLO,CTR BIOTECHNOL,N-0316 OSLO,NORWAY
关键词
Bacillus thuringiensis; Bacillus cereus; classification; genome analysis; pulsed-field gel electrophoresis;
D O I
10.1111/j.1574-6968.1996.tb08379.x
中图分类号
Q93 [微生物学];
学科分类号
071005 ; 100705 ;
摘要
A physical map of the Bacillus thuringiensis subsp. canadensis HD224 chromosome based on Asci, NotI, and SfiI restriction sites has been established. The chromosome map of 4.3 Mb was similar to a revised map of the chromosome of the B. cereus type strain ATCC 14579, except that the B. thuringiensis subsp. canadensis HD224 chromosome lacked a NotI site and had two additional Asci sites. The positions of 27 probes were identical in the common macromap. A probe for the insecticidal toxin gene, cryIA, hybridized only to the B. thuringiensis subsp. canadensis HD224 chromosome. The BssHII ribotype patterns were almost identical confirming the similarity between the two strains.
引用
收藏
页码:163 / 167
页数:5
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