MAG versus PEG: Incorporating a Poly(MAG) Layer to Promote Colloidal Stability of Nucleic Acid/"Click Cluster" Complexes

被引：26

作者：

Buckwalter, Daniel J. ^{[2
]}

Sizovs, Antons ^{[2
]}

Ingle, Nilesh P. ^{[1
]}

Reineke, Theresa M. ^{[1
]}

机构：

[1] Univ Minnesota, Dept Chem, Minneapolis, MN 55455 USA

[2] Virginia Polytech Inst & State Univ, Macromol & Interfaces Inst, Dept Chem, Blacksburg, VA 24061 USA

来源：

ACS MACRO LETTERS | 2012年 / 1卷 / 05期

基金：

美国国家卫生研究院;

关键词：

BETA-CYCLODEXTRIN; GENE DELIVERY; NANOPARTICLES; SIRNA; PDNA; HUMANS; DNA;

D O I：

10.1021/mz300081d

中图分类号：

O63 [高分子化学（高聚物）];

学科分类号：

070305 ; 080501 ; 081704 ;

摘要：

Herein, we demonstrate the reversible addition-fragmentation chain transfer (RAFT) synthesis of an adamantane-conjugated glycopolymer, poly(2-methacrylamido-2-deoxy glucopyranose) (Ad-pMAG), as a hydrophilic coating to promote colloidal stability of click cluster-pDNA complexes in biological media. The Ad-pMAG is assembled via noncovalent interactions through inclusion complex formation between adamantane (Ad) and the beta-cyclodextrin (beta CD) core of the click cluster/pDNA and then further assembled with plasmid DNA to form polyplexes. Ad-pMAG incorporation was favorable over Ad-poly(ethylene glycol) (Ad-PEG) due to the enhanced colloidal stability of the click cluster/pDNA polyplex under physiological salt conditions at high N/P ratios. Interestingly, the uptake and reporter gene expression with polyplexes coated with the Ad-pMAG was much lower in HeLa cells than that observed with two glioma cell lines (U87 and U251 cells) in vitro, possibly indicating some delivery specificity.

引用

页码：609 / 613

页数：5