Expression Stabilities of Candidate Reference Genes for RT-qPCR under Different Stress Conditions in Soybean

被引：59
|
作者：
Ma, Shuhua [1 ]
Niu, Hongwei [1 ]
Liu, Chunji
Zhang, Jie [1 ]
Hou, Chunyan [1 ]
Wang, Dongmei [1 ]
机构：
[1] Agr Univ Hebei, Coll Life Sci, Baoding, Hebei Province, Peoples R China
来源：
PLOS ONE | 2013年 / 8卷 / 10期
基金：
中国国家自然科学基金;
关键词：
REAL-TIME PCR; POLYMERASE-CHAIN-REACTION; SUPERIOR REFERENCE GENES; HOUSEKEEPING GENES; INTERNAL CONTROL; TRANSCRIPT NORMALIZATION; QUANTITATIVE-PCR; RNA EXPRESSION; IDENTIFICATION; VALIDATION;
D O I：
10.1371/journal.pone.0075271
中图分类号：
O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];
学科分类号：
07 ; 0710 ; 09 ;
摘要：
Due to its accuracy, sensitivity and high throughput, real time quantitative PCR (RT-qPCR) has been widely used in analysing gene expression. The quality of data from such analyses is affected by the quality of reference genes used. Expression stabilities for nine candidate reference genes widely used in soybean were evaluated under different stresses in this study. Our results showed that EF1A and ACT11 were the best under salinity stress, TUB4, TUA5 and EF1A were the best under drought stress, ACT11 and UKN2 were the best under dark treatment, and EF1B and UKN2 were the best under virus infection. EF1B and UKN2 were the top two genes which can be reliably used in all of the stress conditions assessed.
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页数：7
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