Transverse-relaxation-optimized (TROSY) gradient-enhanced triple-resonance NMR spectroscopy

被引：85
|
作者：
Loria, JP
Rance, M
Palmer, AG
机构：
[1] Columbia Univ, Dept Biochem & Mol Biophys, New York, NY 10032 USA
[2] Univ Cincinnati, Dept Mol Genet Biochem & Microbiol, Cincinnati, OH 45267 USA
来源：
JOURNAL OF MAGNETIC RESONANCE | 1999年 / 141卷 / 01期
关键词：
TROSY; triple-resonance NMR spectroscopy; triosephosphate isomerase; gradient coherence selection;
D O I：
10.1006/jmre.1999.1891
中图分类号：
Q5 [生物化学];
学科分类号：
071010 ; 081704 ;
摘要：
Two modifications to sensitivity-enhanced gradient-selected TROSY-based triple-resonance NMR experiments are proposed that reduce the overall duration of the pulse sequences and minimize radiation damping effects on water-flipback solvent suppression. The modifications are illustrated for the HNCO-TROSY experiment, but are applicable to all triple-resonance experiments that detect proton magnetization after a reverse polarization transfer step from a N-15 spin. The methods are applied to yeast triosephosphate isomerase, a symmetric dimer with 248 amino acid residues per monomer, (C) 1999 Academic Press.
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页码：180 / 184
页数：5
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