Correlation between type of alkali rinsing, cytotoxicity of bio-nanocellulose and presence of metabolites within cellulose membranes

被引：12

作者：

Junka, Adam ^{[1
]}

Fijalkowski, Karol ^{[2
]}

Zabek, Adam ^{[3
]}

Mikolajewicz, Katarzyna ^{[4
]}

Chodaczek, Grzegorz ^{[4
]}

Szymczyk, Patrycja ^{[5
]}

Smutnicka, Danuta ^{[1
]}

Zywicka, Anna ^{[2
]}

Sedghizadeh, Parish Paymon ^{[6
]}

Dziadas, Mariusz ^{[7
]}

Mlynarz, Piotr ^{[3
]}

Bartoszewicz, Marzenna ^{[1
]}

机构：

[1] Wroclaw Med Univ, Dept Pharmaceut Microbiol & Parasitol, Borowska 211A, PL-50556 Wroclaw, Poland

[2] West Pomeranian Univ Technol, Dept Immunol Microbiol & Physiol Chem, Piastow 45, PL-70311 Szczecin, Poland

[3] Wroclaw Univ Technol, Dept Bioorgan Chem, Fac Chem, Wyspianskiego 27, PL-50534 Wroclaw, Poland

[4] Wroclaw Res Ctr EIT, Confocal Microscopy Lab, Stablowicka 147, PL-54066 Wroclaw, Poland

[5] Wroclaw Univ Technol, Fac Mech Engn, Ctr Adv Mfg Technol CAMT FPC, Lukasiewicza 5, PL-50371 Wroclaw, Poland

[6] Univ Southern Calif, Ostrow Sch Dent, Ctr Biofilms & Craniofacial Mol Biol, Los Angeles, CA USA

[7] Wroclaw Med Univ, Dept Bromatol & Dietet, Borowska 211A, PL-50556 Wroclaw, Poland

来源：

CARBOHYDRATE POLYMERS | 2017年 / 157卷

关键词：

Alkali rinsing; Bacterial nanocellulose; Metabolite; Cytotoxicity; Macrophage; BACTERIAL-CELLULOSE; GLUCONACETOBACTER-HANSENII; AGITATED CULTURE; CELLS; WASTE;

D O I：

10.1016/j.carbpol.2016.10.007

中图分类号：

O69 [应用化学];

学科分类号：

081704 ;

摘要：

The study aimed at evaluation of various types of alkali rinsing with regard to their efficacy in terms of removal, not only of bacteria but also bacterial metabolites, from cellulose matrices formed by three IComagataeibacterxylinus strains. Moreover, we tested the type of alkali rinsing on membrane cytotoxicity in vitro in fibroblast and osteoblast cells and we compared matrices' ability to induce oxidative stress in macrophages. We identified 11 metabolites of bacterial origin that remained in cellulose after rinsing. Moreover, our results indicated that the type of alkali rinsing should be adjusted to specific K. xylinus strains that are used as cellulose producers to obtain safe biomaterials in the context of low cytotoxicity and macrophage induction. The findings have translational importance and may be of direct significance to cellulose dressing manufacturers. (C) 2016 Elsevier Ltd. All rights reserved.

引用

页码：371 / 379

页数：9