A lipid core peptide construct containing a conserved region determinant of the group a streptococcal M protein elicits heterologous opsonic antibodies

被引：55
|
作者：
Olive, C
Batzloff, MR
Horváth, A
Wong, A
Clair, T
Yarwood, P
Toth, I
Good, MF
机构：
[1] Queensland Inst Med Res, Div Infect Dis & Immunol, Cooperat Res Ctr Vaccine Technol, Brisbane, Qld 4029, Australia
[2] Univ Queensland, Sch Pharm, Brisbane, Qld 4072, Australia
来源：
INFECTION AND IMMUNITY | 2002年 / 70卷 / 05期
关键词：
D O I：
10.1128/IAI.70.5.2734-2738.2002
中图分类号：
R392 [医学免疫学]; Q939.91 [免疫学];
学科分类号：
100102 ;
摘要：
The study reported here investigated the immunogenicity and protective potential of a lipid core peptide (LCP) construct containing a conserved region determinant of M protein, defined as peptide J8. Parenteral immunization of mice with LCP-J8 led to the induction of high-titer serum immunoglobulin G J18-specific antibodies when the construct was coadministered with complete Freund's adjuvant (CFA) or administered alone. LCP-J8 in CFA had significantly enhanced immunogenicity compared with the monomeric peptide J8 given in CFA. Moreover, LCP-J8/CFA and LCP-J8 antisera opsonized four different group A streptococcal (GAS) strains, and the antisera did not cross-react with human heart tissue proteins. These data indicate the potential of an LCP-based M protein conserved region GAS vaccine in the induction of broadly protective immune responses in the absence of a conventional adjuvant.
引用
收藏
页码：2734 / 2738
页数：5
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