A proteomic study on the protective effect of kaempferol pretreatment against deoxynivalenol-induced intestinal barrier dysfunction in a Caco-2 cell model

被引：5
|
作者：
Wang, Xiaojie [1 ]
Li, Li [1 ]
Zhang, Genyi [1 ]
机构：
[1] Jiangnan Univ, Sch Food Sci & Technol, State Key Lab Food Sci & Technologry, Wuxi 214122, Jiangsu, Peoples R China
来源：
FOOD & FUNCTION | 2020年 / 11卷 / 08期
关键词：
EXPRESSION; INTEGRITY; MODULATION; PERMEABILITY; TOXICOLOGY; CLAUDIN-4; JUNCTIONS; EXPOSURE; STRESS; ROLES;
D O I：
10.1039/d0fo01579b
中图分类号：
Q5 [生物化学]; Q7 [分子生物学];
学科分类号：
071010 ; 081704 ;
摘要：
Deoxynivalenol (DON) is one of the most widely distributed mycotoxins in the food chain, and the protective effect of kaempferol (KAM) pretreatment against the barrier dysfunction induced by DON in a Caco-2 cell model was investigated using a proteomic approach. The results showed that after KAM pretreatment, both the numbers of down- and up-regulated differentially expressed proteins were significantly lower than those in the DON group. Gene ontology analysis revealed that differentially expressed proteins were enriched in cell adhesion molecule binding, cell junction, and cell junction assembly. Further study demonstrated that KAM pretreatment affected the expression and assembly of tight junction and adherens junction proteins through the PKA pathway and MAPK/ERK pathway to improve the barrier integrity of the Caco-2 cell monolayer, and nutraceutical approach based on bioactive phytochemicals to improve the body's immunity might be an effective strategy to combat the adverse effects of mycotoxins on intestinal barrier function.
引用
收藏
页码：7266 / 7279
页数：14
相关论文
共 50 条
  • [1] A proteomic study on the protective effect of kaempferol pretreatment against deoxynivalenol-induced intestinal barrier dysfunction in a Caco-2 cell model
    Wang, Xiaojie
    Li, Li
    Zhang, Genyi
    Zhang, Genyi (genyiz@gmail.com), 1600, Royal Society of Chemistry (11): : 7266 - 7279
  • [2] Protective Capacity of Resveratrol, a Natural Polyphenolic Compound, against Deoxynivalenol-Induced Intestinal Barrier Dysfunction and Bacterial Translocation
    Ling, Ka-Ho
    Wan, Murphy Lam Yim
    El-Nezami, Hani
    Wang, Mingfu
    CHEMICAL RESEARCH IN TOXICOLOGY, 2016, 29 (05) : 823 - 833
  • [3] Weissella cibariaAttenuated LPS-Induced Dysfunction of Intestinal Epithelial Barrier in a Caco-2 Cell Monolayer Model
    Huang, Liping
    Cui, Kang
    Mao, Wenhao
    Du, Yurong
    Yao, Ning
    Li, Zhen
    Zhao, Huan
    Ma, Wang
    FRONTIERS IN MICROBIOLOGY, 2020, 11
  • [4] Synergistic Protective Effect of Konjac Mannan Oligosaccharides and Bacillus subtilis on Intestinal Epithelial Barrier Dysfunction in Caco-2 Cell Model and Mice Model of Lipopolysaccharide Stimulation
    Chen, Lupeng
    Zhang, Shuai
    Wu, Shi
    Ren, Zhuqing
    Liu, Guoquan
    Wu, Jian
    FRONTIERS IN IMMUNOLOGY, 2021, 12
  • [5] Protective effect of various polyphenols against mitochondrial dysfunction induced by indomethacin in caco-2 cells
    Carrasco-Pozo, C.
    Speisky, H.
    Gotteland, M.
    ANNALS OF NUTRITION AND METABOLISM, 2011, 58 : 82 - 82
  • [6] Effects of butyrate on intestinal barrier function in a Caco-2 cell monolayer model of intestinal barrier
    Peng, Luying
    He, Zhenjuan
    Chen, Wei
    Holzman, Ian R.
    Lin, Jing
    PEDIATRIC RESEARCH, 2007, 61 (01) : 37 - 41
  • [7] EFFECTS OF INULIN ON INTESTINAL BARRIER FUNCTION IN A CACO-2 CELL MONOLAYER MODEL OF INTESTINAL BARRIER
    Skinner, A. G.
    Tucker, Christopher
    Fatima, Aiman
    Siddiqui, M. R.
    Singh, Vineet
    Akhtar, Suhail
    GASTROENTEROLOGY, 2022, 162 (07) : S460 - S460
  • [8] Effects of Butyrate on Intestinal Barrier Function in a Caco-2 Cell Monolayer Model of Intestinal Barrier
    Luying Peng
    Zhenjuan He
    Wei Chen
    Ian R Holzman
    Jing Lin
    Pediatric Research, 2007, 61 : 37 - 41
  • [9] Mitigation effect of hesperidin on X-ray radiation-induced intestinal barrier dysfunction in Caco-2 cell monolayers
    Park, Ha -Young
    Yu, Jin-Hee
    FOOD AND CHEMICAL TOXICOLOGY, 2024, 186
  • [10] The Effect of Prim-O-Glucosylcimifugin on Tryptase-Induced Intestinal Barrier Dysfunction in Caco-2 Cells
    Xu, Lu
    Cai, Jieyi
    Tian, Aofei
    Qian, Kai
    Qin, Renan
    Qi, Shaoyun
    Tan, Xupeng
    Qiu, Yuqin
    Gong, Mengjuan
    Han, Bin
    Hu, Xugang
    BIOLOGICAL & PHARMACEUTICAL BULLETIN, 2018, 41 (09) : 1355 - 1361
12345