Purification and identification of flavonoids in Portualca olearcea by macroporous adsorption resin and HPLC

Portulaca oleracea is a kind of food and medicinal plant in China. Flavonoids are the main active ingredients of the medicinal function of this plant. In this study, the macroporous adsorption resins (MARs) were employed to purify flavonoids from the crude extract of P. oleracea. The properties of six kinds of common used MARs were evaluated. The results showed that HPD600 had higher separation efficiency. The separation parameters were also optimized according to dynamic adsorption and desorption experiments. In addition, a high-performance liquid chromatography (PLC) method had been performed and validated for flavonoids analysis in P. oleracea. The results showed that quercetin, kaempferol, luteolin, and apigenin were the main component of flavonoids compounds of P. oleracea. This study is expected to provide useful help for the purification and application of flavonoids of P. oleracea.