Identification and characterization of an LCAT-like Arabidopsis thaliana gene encoding a novel phospholipase A

被引：17

作者：

Chen, Guanqun ^{[1
]}

Greer, Michael S. ^{[1
]}

Lager, Ida ^{[2
]}

Yilmaz, Jenny Lindberg ^{[3
]}

Mietkiewska, Elzbieta ^{[1
]}

Carlsson, Anders S. ^{[2
]}

Stymne, Sten ^{[2
]}

Weselake, Randall J. ^{[1
]}

机构：

[1] Univ Alberta, Dept Agr Food & Nutr Sci, Agr Lipid Biotechnol Program, Edmonton, AB T6G 2P5, Canada

[2] Swedish Univ Agr Sci, Dept Plant Breeding & Biotechnol, Alnarp, Sweden

[3] Scandinavian Biotechnol Res ScanBiRes AB, Alnarp, Sweden

来源：

FEBS LETTERS | 2012年 / 586卷 / 04期

基金：

加拿大自然科学与工程研究理事会;

关键词：

Hydrolase; Phospholipase A; Arabidopsis thaliana; Saccharomyces cerevisiae; DIACYLGLYCEROL ACYLTRANSFERASE; ENZYME; CATALYZES; PLANTS; YEAST; ACID;

D O I：

10.1016/j.febslet.2011.12.034

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

A previously uncharacterized Arabidopsis lecithin: cholesterol acyltransferase (LCAT) family gene (At4g19860) was functionally expressed in yeast, where it was demonstrated to encode a novel cytosolic and calcium-independent phospholipase A with preferences for the sn-2 position. This enzyme shows optimal activity at pH 5.0, exhibits a headgroup specificity for phosphatidylcholine > phosphatidic acid > phosphatidylethanolamine > phosphatidylglycerol > phosphatidylserine and has an acyl chain specificity for oleoyl > linoleoyl > ricinoleoyl. The expression of AtLCAT-PLA inhibited yeast cell growth and fatty acid accumulation. AtLCAT-PLA transcript in Arabidopsis was detected at high levels in roots and siliques. (C) 2012 Federation of European Biochemical Societies. Published by Elsevier B. V. All rights reserved.

引用

页码：373 / 377

页数：5

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