Development of a Multiplex Polymerase Chain Reaction Method for cps Typing of Actinobacillus pleuropneumoniae Serovars 1, 2, 5, 7, and 15

被引：4

作者：

Ito, Hiroya ^{[1
]}

Sueyoshi, Masuo ^{[2
]}

机构：

[1] Natl Agr & Food Res Org, Natl Inst Anim Hlth, Tsukuba, Ibaraki 3050856, Japan

[2] Miyazaki Univ, Ctr Anim Dis Control, Miyazaki, Miyazaki 8892192, Japan

来源：

JARQ-JAPAN AGRICULTURAL RESEARCH QUARTERLY | 2015年 / 49卷 / 03期

关键词：

pig; pleuropneumonia; serotyping; ANTIMICROBIAL SUSCEPTIBILITY; PCR ASSAY; IDENTIFICATION; SEROTYPE-2;

D O I：

10.6090/jarq.49.277

中图分类号：

S [农业科学];

学科分类号：

09 ;

摘要：

We developed a multiplex polymerase chain reaction (PCR) method using primers specific to the capsular polysaccharide biosynthesis genes (cps) to type Actinobacillus pleuropneumoniae serovars 1, 2, 5, 7, and 15. This multiplex PCR method may be useful for the typing of serovar 15, which is recently becoming more prevalent, as well as the most prevalent serovars 1, 2, 5, and 7 in Japan.

引用

页码：277 / 280

页数：4

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