New PCR assay for rapid and quantitative detection of human cytomegalovirus in cerebrospinal fluid

被引：17
|
作者：
Vogel, JU
Cinatl, J
Lux, A
Weber, B
Driesel, AJ
Doerr, HW
机构：
[1] UNIV FRANKFURT,INST MED VIROL,D-60596 FRANKFURT,GERMANY
[2] UNIV ESSEN GESAMTHSCH KLINIKUM,INNERE KLIN TUMOR FORSCH,D-45122 ESSEN,GERMANY
[3] TUV HESSEN,D-65760 ESCHBORN,GERMANY
来源：
JOURNAL OF CLINICAL MICROBIOLOGY | 1996年 / 34卷 / 02期
关键词：
D O I：
10.1128/JCM.34.2.482-483.1996
中图分类号：
Q93 [微生物学];
学科分类号：
071005 ; 100705 ;
摘要：
Rapid Chelex extraction combined with an automated hybridization assay for the detection of PCR-amplified human cytomegalovirus DNA from cerebrospinal fluid was established. Quantitation of DNA was performed with a plasmid being used as an external standard. The detection limit was 10 copies per mu l. Quantitative detection of human cytomegalovirus DNA could be achieved over a range from 10 to 10(4) copies per mu l.
引用
收藏
页码：482 / 483
页数：2
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