Thermo-Sensitive PLGA-PEG-PLGA Tri-Block Copolymer Hydrogel as Three-Dimensional Cell Culture Matrix for Ovarian Cancer Cells

被引：14
|
作者：
Zhou, Naizhen [1 ]
Hu, Ke [2 ]
Guo, Zhaobin [1 ]
Zhang, Qiying [1 ]
Chen, Junsong [1 ]
Zhang, Tianzhu [1 ,3 ]
Gu, Ning [1 ,3 ]
机构：
[1] Southeast Univ, Sch Biol Sci & Med Engn, Natl Demonstrat Ctr Expt Biomed Engn Educ, Jiangsu Key Lab Biomat & Devices,State Key Lab Bi, Nanjing 210096, Jiangsu, Peoples R China
[2] Nanjing Med Univ, Sch Basic Med Sci, Dept Biomed Engn, Key Lab Clin & Med Engn, Nanjing 211166, Jiangsu, Peoples R China
[3] Collaborat Innovat Ctr Suzhou Nanosci & Technol, Suzhou Key Lab Biomat & Technol, Suzhou 215123, Peoples R China
来源：
JOURNAL OF NANOSCIENCE AND NANOTECHNOLOGY | 2018年 / 18卷 / 08期
基金：
中国国家自然科学基金;
关键词：
Thermo-Sensitive Hydrogel; PLGA-PEG-PLGA; 3D Cell Culture; Ovarian Cancer Cell; DELIVERY; CHITOSAN; PLATFORM; WATER;
D O I：
10.1166/jnn.2018.15372
中图分类号：
O6 [化学];
学科分类号：
0703 ;
摘要：
Thermo-sensitive hydrogels which could encapsulate cells and provide a three dimensional (3D) microenvironment have great potential in building new cell culture models in vitro. In this study, a thermal responsive hydrogel based on PLGA-PEG-PLGA tri-block copolymers was developed as matrix for 3D ovarian cancer cell culturing. The gelation of PLGA-PEG-PLGA tri-block copolymer was concentration-dependent. SEM images showed the pores were suitable for the formation of 3D cell structures. Cell morphological results showed that large aggregates of ovarian cancer cells (HO8910) were formed after cultured for 10 days. Therefore, hydrogel based on PLGA-PEG-PLGA tri-block copolymers hold potential as in vitro cell culture matrix for ovarian cancer cells.
引用
收藏
页码：5252 / 5255
页数：4
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