Cryopreservation of alginate coated shoot tips of in vitro grown Holostemma annulare (Roxb.) K. Schum, an endangered medicinal plant:: Influence of preculture and DMSO treatment on survival and regeneration

被引：0

作者：

Decruse, SW ^{[1
]}

Seeni, S ^{[1
]}

Pushpangadan, P ^{[1
]}

机构：

[1] Trop Bot Garden & Res Inst, Plant Biotechnol Div, Thiruvananthapuram 695562, Kerala, India

来源：

CRYOLETTERS | 1999年 / 20卷 / 04期

关键词：

Holostemma annulare; cryopreservation; shoot tips; medicinal plants; DMSO; encapsulation-dehydration;

D O I：

暂无

中图分类号：

Q [生物科学];

学科分类号：

07 ; 0710 ; 09 ;

摘要：

Shoot tips isolated from preconditioned shoot cultures of Holostemma annulare, an endangered medicinal plant of the Indian sub-continent were successfully cryopreserved after encapsulation, dehydration and preculture in the dark in modified MS medium containing 0.5-0.75 M sucrose and 3-5% DMSO. Preculture in sucrose alone for 2-3 days was sufficient to obtain 46-56% survival and 42-52% shoot regeneration rates from the cryopreserved shoot tips. A 3-day preculture with 3 and 5% DMSO enhanced the survival rates to 72-76% and regeneration rates to 67-68% but all of the enhancement came from cells producing callus. Shoot regenerations from these shoot tips precultured with 3 and 5% DMSO occurred only at 54 and 30% rates respectively. Prolonged preculture (> 3 days) in presence of DMSO reduced the survival of shoot tips exposed to LN.

引用

页码：243 / 250

页数：8