STUDY OF BIOLOGICAL SAMPLES WITH DETECTOR MEDIPIX2

The microradiography is an imaging method, in which roentgen radiation passes through a biological sample and so visualize its inner structure on detection surface. The roentgen transmission radiography is based on the attenuation of X-rays in matter. The X-ray is partly absorbed by scanned sample and the rest is detected by suitable detector. When attenuation of radiation is not equal, outgoing radiation has different intensity and contains latent screen information of scanned sample. Soft tissues do not attenuate Xrays a lot so the difference between them is poorly visible. Currently used imaging detectors as radiography film or scintillator detectors, cannot visualize required information about inner structure of scanned sample. While semiconductor pixel detectors count single particle of radiation and because of theirs non-limited dynamic range are appropriate for imagining of soft tissues by X-ray radiation. These features of detectors confer high quality and high contrast. The measuring microradiographic setup developed at our institute, allows not only static but also dynamic observation and documentation of biological processes in real time. As a source of roentgen radiation we use X-ray tube FeinFocus with microfocus that way we are able to visualize objects with resolution downto 1 um. The implementation of detector Medipix2 brings new possibilities in non-invasive imaging of living objects.