Toll-like receptor signaling is functional in immune cells of the endangered Tasmanian devil

被引：14

作者：

Patchett, Amanda L. ^{[1
]}

Latham, Roger ^{[2
]}

Brettingham-Moore, Kate H. ^{[2
]}

Tovar, Cesar ^{[1
]}

Lyons, A. Bruce ^{[2
]}

Woods, Gregory M. ^{[1
,2
]}

机构：

[1] Univ Tasmania, Menzies Inst Med Res, Hobart, Tas 7000, Australia

[2] Univ Tasmania, Sch Med, Hobart, Tas, Australia

来源：

DEVELOPMENTAL AND COMPARATIVE IMMUNOLOGY | 2015年 / 53卷 / 01期

基金：

澳大利亚研究理事会;

关键词：

Tasmanian devil; Toll-like receptor; Devil facial tumour disease; Adjuvant; Innate immunity; Marsupial; FACIAL-TUMOR DISEASE; I-KAPPA-B; SARCOPHILUS-HARRISII; DENDRITIC CELLS; CANCER CELLS; CPG-DNA; ACTIVATION; APOPTOSIS; RESPONSES; EXPRESSION;

D O I：

10.1016/j.dci.2015.07.003

中图分类号：

S9 [水产、渔业];

学科分类号：

0908 ;

摘要：

Devil facial tumour disease (DFTD) is a fatally transmissible cancer that threatens the Tasmanian devil population. As Tasmanian devils do not produce an immune response against DFTD cells, an effective vaccine will require a strong adjuvant. Activation of innate immune system cells through toll-like receptors (TLRs) could provide this stimulation. It is unknown whether marsupials, including Tasmanian devils, express functional TLRs. We isolated RNA from peripheral blood mononuclear cells and, with PCR, detected transcripts for TLRs 2, 3, 4, 5, 6, 7, 8, 9, 10 and 13. Stimulation of the mononuclear cells with agonists to these TLRs increased the expression of downstream TLR signaling products (IL1 alpha, IL6, IL12A and IFN beta). Our data provide the first evidence that TLR signaling is functional in the mononuclear cells of the Tasmanian devil. Future DFTD vaccination trials will incorporate TLR agonists to enhance the immune response against DFTD. Crown Copyright (C) 2015 Published by Elsevier Ltd. All rights reserved.

引用

页码：123 / 133

页数：11

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